Fig. 4. Midgut migration is entirely dependent on integrins. Wild-type and mutant embryos were stained with anti-filamin 1 (Cheerio) antibodies to mark the migrating midgut endoderm (A'-H' and magenta in A-H) and anti-Fasciclin 3 antibodies to label the visceral mesoderm palisade (green in A-H) upon which the midgut cells migrate. Genotypes are labelled above and stages are indicated on the left. All mutant embryos lack both the maternal and zygotic contributions of the indicated gene products. (A,B) While the midgut primordia have nearly met in the centre of stage 12 wild-type embryos, migration is delayed in embryos lacking ßPS. (F) ßPS^- mutant embryos eventually recover and midgut migration is complete by stage 13. (G) Embryos lacking ßPS and ß do not recover, however, and midgut migration is completely blocked. (D) Midgut migration is delayed in embryos lacking talin but, like ßPS^- mutants, migration is complete by stage 13 (H).