Fig. 6. ßPS is required for talin localization in the midgut. Wild-type and mutant embryos deficient for both maternal and zygotic ß subunits were stained with anti-talin and anti-filamin 1 antibodies. (A) Talin (A' green) and filamin 1 (A'' red) are concentrated at the interface between the midgut endoderm (en) and the visceral mesoderm (vm). Filamin 1 is expressed in the endoderm while talin is expressed in both tissues. (B,C) In the absence of maternal and zygotic ßPS, talin is diffuse throughout the cytoplasm in both the endoderm and mesoderm (B') and is not altered by the additional absence of ß (C'). Filamin 1 localization to the basal surface of the endoderm is not altered by the absence of ßPS (B'') or by the absence of both ßPS and ß (C'').