Fig. 3. Dfr expression can be reduced in the ISNb motoneurons. (A) Wild-type Dfr expression (green) in the ventral nerve cord of a stage 16 dfr^B129 UAS-dsdfr/+transgenic embryo. Dfr is expressed in the ISNb neurons, including the RP neurons (lower arrow). Slit expression (magenta) is used to mark the Dfr-expressing midline glia (white/colocalization). (B) Dfr expression (green) is severely reduced or eliminated in Lim3B-Gal4;dfr^B129 UAS-dsdfr/+ embryos. The absence of Dfr labeling in the RP neurons is indicated by the arrows. Dfr expression remains in the Slit-expressing midline glia (arrowhead) as well as in lateral neuronal clusters. (C) Two neuromeres of a Lim3B-Gal4;UAS-taumycEGFP VNC labeled with GFP (green) and the Lim3 antibody (magenta). Lim3 expression is co-localized with the reporter GFP expression in the RP neurons (lower arrow) and the TN neurons (arrowhead). The process of the TMNp neuron is also visualized in this image (upper arrow).