Fig. 5. Dfr misexpression in the TN neurons results in a redirection of TN motor axons to ISNb muscle targets. (A and B1) In the wild-type embryo, two TN motoneurons leave the VNC. One, TMN 25, innervates ventral muscle 25 (out of the plane of focus) and the other, TMNp, contacts the ventral process of the LBD neuron. The TN and ISNb fascicles do not ever meet and are on different focal planes (arrowhead). All embryos are stained with anti-Fas2 antibodies. (B) Schematic summary of wild-type and Dfr misexpression phenotypes in the TN neurons (blue). (B2) When Dfr is misexpressed in the TN neurons, the TMNp motor axons target the ISNb muscle field. (C,D) In Lim3B-Gal4;UAS-dfr embryo late stage 16 embryos, the TN axons have retargeted to either enter or innervate the ISNb muscle field (arrows).