Fig. 10. Osteoporosis in rs/rs adult mice. (A-F) X-ray radiography (A,B) and histology (C-F) of the lumbo-sacral vertebrae were carried out in rs/rs (B,D,F) and control (A,C,E) animals. The boxed areas in C and D are shown at a higher magnification in E and F, respectively. The arrowhead in F points to a group of chondrocytes inside the trabecule. Scale bars: 50 µm. (G-I) Low bone mass phenotype in rs mutants demonstrated by pQCT. Bone density and the thickness of cortical bones in the metaphysis of the tibia were measured in rs/rs, rs/+ and +/+ animals (14 month-old females in triplicate for each genotype), and results are graphically depicted for bone density measures from total tibial metaphysis (G) and the cortex part (H), and for the cortical bone thickness (I). Values represent the means + standard errors. Single and double asterisks indicate statistically significant reductions (P<0.05 and P<0.01, respectively) in rs/rs compared with +/+.