Fig. 5. Basic FGF and LIF synergistically suppress the expression of MHC. (A-D) Single myofibers isolated from gastrocnemius muscle were cultured in pmGM (A), or in pmGM supplemented with bFGF alone (B), LIF alone (C), or bFGF plus LIF (D), for 6 days. Cells were then subjected to immunostaining for MHC (red). Nuclei were visualized by DAPI. Scale bars: 100 µm. (E) Quantitative analysis of inhibition of MHC expression by growth factors. Percentages of nuclei in MHC-expressing cells to total nuclei (bars) and total numbers of nuclei in each field (0.55 mm²) (open circles) were calculated in four independent samples. Averages and standard errors are shown.