Fig. 2. Overexpression of Dx ectopically activates the target genes of N signaling. (A-C) Within the Dx-overexpressing clones (green), vgBE (purple) was activated in a cell autonomous manner. C is a merged image of A and B. (D-F) Within cells overexpressing Dx (green), the wg promoter was ectopically activated. The promoter activity of wg was detected by wg-lacZ (purple). F is a merged image of D and E. (G-I) Within cells overexpressing Dx (green), the Dl promoter was ectopically activated. The promoter activity of Dl was detected by Dl-lacZ (purple). I is a merged image of G and H. Weak cell non-autonomous induction of Dl-lacZ was also observed. (J,L) Expression of N in wild-type wing discs. N was detected by an anti-N^ICD antibody (green). (K,L) Activation of the N promoter was visualized by N-lacZ (purple). (M-O) Within the Dx-overexpressing clones (green), N-lacZ (purple) was activated in a cell autonomous manner. O is a merged image of M and N. (P-R) Within the N^ICD-overexpressing clones (green), N-lacZ (purple) was activated cell autonomously. R is a merged image of P and Q. All images were obtained from third-instar wing discs with respective genetic manipulation.