Development -- Hatakeyama et al. 131 (22): 5539 Figure IG3

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Fig. 3. Cell death and dorso-ventral patterning of the spinal cord. (A-F) TUNEL assay indicates that cell death (green) is not increased in Hes1^–/–Hes5^–/– spinal cord (D-F). (G-P) In-situ hybridization analysis of Shh for the floor plate (G,L), Sim1 for V3 interneurons (H,M), Evx1 for V0 interneurons (I,N), Lhx2 for D1A interneurons (J,O), and Wnt1 for the roof plate (K,P) at E10.5. Except for the hypomorphic roof plate, the dorso-ventral patterning is mostly normal in Hes1^–/–Hes5^–/– spinal cord (L-P). Scale bars: 100 µm.