Fig. 7. Eya1 controls Six1 expression in epibranchial placodes. (A,B,F,G) Transverse sections showing Eya1 expression in distal VIIth placode (VIIp, A) at E9.0 and distal IXth placode (pIX, B) at E9.0 and its expression in these structures was preserved in Six1^–/– embryos (F,G). (C,D,H,I) Transverse sections showing Six1^lacZ expression in distal VIIth, IXth and Xth placodal, migratory (arrowhead) and aggregating neuronal precursors in the ganglion anlagen (gVII, gIX, gX) at E9.25-9.5 in Six1^lacZ/+ heterozygous embryos (C,D,H) and its expression was undetectable in Eya1^–/– epibranchial neuronal precursors (I). (E,J) Bmp7 expression in the pharyngeal pouches (p1-p3, E) at E10.5 was unaffected in Eya1^–/– embryos (J).