Fig. 4. The Gli2^–/– phenotype is specific to the EGL. Antibody marker analysis shows that Purkinje cells marked with Calbindin (A,E) and Bergmann glia marked with BLBP (B,F) are present and their general cellular organization appears normal in Gli2^–/– embryos at E18.5. Gli-lacZ is not detectable in Gli2^–/– (compare C and G). However, Gli mRNA is detectable by RNA in-situ hybridization in Gli2^–/– embryos (H), but its levels are much weaker than in WT (D). Anterior is to the left. Scale bar: 100 µm.