Fig. 5. Shh-P1 mutants have a larger but well patterned cerebellum. Dorsal views of adult mutant brains (E) show the transgenic cerebellum is much larger than the WT (A). Cresyl Violet staining of paraffin sections of adult mutant cerebella shows enlargement of the cerebellum and a thicker IGL (indicated by bar) in medial (F) and lateral (G) sections, compared with WT (B,C). In the vermis, the phenotype is more severe in lobes III, IV, IV and IX and around the primary fissure. At P0 (inset, D,H) and P5, Gli-lacZ expression is maintained in its normal pattern in Shh-P1 mutants (H), although at higher levels than in WT (D). In sections, anterior is to the left. Scale bar: 400 µm in B,C,F,G; 250 µm in D,H.