Fig. 7. The Shh-P1 EGL is not thicker at early stages, but persists longer than in WT cerebella. Antibody staining for PCNA at P5, a marker for proliferating cells, appears similar in WT (A) and mutant (E) EGL. Cresyl Violet staining of P14 sections shows the EGL is one cell layer thick in WT (B), and three to four cell layers thick in mutants (F). PCNA labeling shows the presence of proliferating cells in the EGL at P14 in both WT and mutant (C,G). By P16, the EGL has been depleted in WT (D), but one cell layer is still present in the mutant (H). Red dashed line indicates division between two lobes. Scale bar: 50 µm.