Fig. 6. Pax6 is required for ngn1 and transgene expression in the diencephalon of the zebrafish embryo. (A-F) Control (A,C,E) and morpholino-injected (B,D,F) embryos hybridised to ngn1 (A-D) and gfp (E,F) antisense probes. Expression of the endogenous ngn1 mRNA and the transgene (–8.4ngn1:gfp) is reduced in the diencephalon (arrowheads) but not in the telencephalon (t). Embryos were injected with a cocktail of morpholino oligonucleotides directed against pax6.1 and pax6.2. Embryos are 28 hpf. (A,B) Dorsal views of embryos with anterior oriented upwards; (C-F) lateral views. e, eye.