Fig. 7. The LATE region is conserved between tetrapods and zebrafish. (A) Sequence comparison of the LATE regulatory region of zebrafish ngn1 with that of human, mouse, chicken and Xenopus tropicalis. The aligned sequences comprise nucleotides –1775 and –1368 in the zebrafish ngn1 genomic regulatory region. In each column, the nucleotides are printed on a black background when they are all identical, or on grey background when one base is present in more than half of the sequences. Boxed regions indicate regions where the zebrafish sequence is similar to the Pax6 consensus-binding site (Epstein et al., 1994). Three sites (A, B and C) were scored. (B) Comparison of the three putative Pax6-binding sites with the consensus-binding sequence.