Fig. 8. Pax6 binds to site-C of the LATE region. Electromobility-shift assays with recombinant mouse Pax6 protein. Radioactively labelled oligonucleotide containing site-C of the LATE region was incubated with Pax6 protein without competitor oligonucleotide, or with a 50-fold molar excess of cold site-C oligonucleotide (sC), or with an oligonucleotide that contained a cluster of point mutations (msC) in the Pax6-binding site homology domain. The site-C competitor abolished the shifted band, whereas the complex formation was not affected by the presence of the mutated oligonucleotide, demonstrating that the interaction of Pax6 protein is dependent on an intact Pax6-binding site. As a positive control, an oligonucletide (Control) harbouring the Pax6-binding site described by Czerny et al. (Czerny et al., 1993) was used.