Fig. 3. SEM images and specific expression patterns of DL and OSH1. (A) Wild-type flower at initiation of stamen primordia. (B) Wild-type flower that has started to form two styles in the pistil. (C) Almost-mature wild-type flower, in which stigmatic hairs are about to begin to grow on the styles. (D) fon1-2 flower. The floral meristem is enlarged along the palea-lemma axis. Additional stamens form in the same whorl as original stamens, and palea/lemma-like organs form ectopically on the lateral side of the meristem. (E) fon1-2 flower that develops two ectopic palea-like organs side by side. (F) fon1-2 flower that develops ectopic stamens in two whorls. (G) fon1-2 flower that has an enlarged floral meristem and develops two sets of carpels in an alternate phyllotaxy. (H,I) fon1-2 flowers that produce a number of pistils. (J) Close-up of the central region that remains undifferentiated in almost-mature fon1-2 flowers. (K) A secondary floret produced in fon1-2. The secondary flower (large arrow) has a few stamens and a carpel subtended with palea and lemma (the palea is removed to show the inside of the secondary floret). (L) Partial loss of stamen identity in fon1-2. Stigmatic hairs (black arrow) are produced at the top of the stamen instead of the anther and an undifferentiated cell mass has formed at the base of the filament (white arrow). (M,N) Spatial expression of DL. (M) Wild type; (N) fon1-2. Three sets of carpels develop in fon1-2. (O,P) Spatial expression of OSH1. (O) Wild type; (P) fon1-2. Whereas OSH1 expression disappears after carpel development in wild type, its expression is maintained in the floral meristem in the center of the flower in fon1-2. ca, carpel; eca, ectopic carpel; epl, ectopic palea/lemma-like organ; est, ectopic stamen that develops in an additional third whorl; fm, floral meristem; le, lemma; pa, palea; st, stamen; st2, stamen that develops in the secondary flower; sty, style. Scale bars: 100 µm in A-J,M-P; 500 µm in K,L.