Fig. 3. BMP inhibits late markers of neural induction. BMP4 was electroporated into the prospective neural plate at stage 3+ and the consequences analysed in time course. After 12 hours and 15 hours of incubation, the early marker Sox3 is not affected (A,B,G,H), while the later marker Sox2 is strongly downregulated in the neural plate (C,D,I,J). By 20 hours after electroporation, both Sox3 (M,N) and Sox2 (O,P) are downregulated. Neither Sox3 nor Sox2 is altered when control GFP is electroporated (E,F,K,L,Q,R).