Fig. 5. BMP inhibition in combination with FGF (FGF2, FGF3 or FGF4) and Wnt antagonists does not induce neural tissue directly. (A,B,I,J,Q,R) Beads with FGF2, FGF3 or FGF4 protein cannot induce either brachyury (light blue) or Sox2 (purple). (C-H,K-P) Likewise, FGF2 or FGF3 together with Smad6 and/or Wnt antagonists does not induce brachyury or Sox2. (S-U) Misexpression of Smad6 and FGF4 induces both brachyury and Sox2; (V-X) addition of Wnt antagonists to the combination inhibits induction of both markers.