Fig. 2. Repression of hth, but not tsh, requires Dpp signaling. (A-D) Adult wings containing multiple Med^adro clones induced in a Minute background by vgBE::flp. Mutant clones are marked by y- tissue, and denoted by black arrows. (A) Med^adro clones result in reduced growth of the wing blade and hinge along the PD axis. (B) A mutant Med^adro clone sorts out from surrounding wild-type tissue in the wing blade. (C) A mutant Med^adro clone differentiates wing margin bristles and non-autonomously induces wing margin elements in adjacent wild-type cells. (D) Med^adro clones located near the anteroposterior (AP) compartment boundary of the hinge differentiate bristles characteristic of the lateral hinge. (E-U) Immunostains of wing discs; Hth staining is in blue, Tsh is in red, and clones are marked by the absence of CD2 in green. (E-H) Late third instar wing disc containing Med^adro M+ clones induced by vgBE::flp. (F-H) Magnification of the region denoted by the white box in E. A large mutant clone in the wing pouch ectopically expresses hth, but not tsh. (I-K) Late third instar wing disc containing Med^adro M+ clones induced by heat shock in the mid-third instar. (J,K) Magnification of the region denoted by the white box in I. The yellow arrowheads point to ectopic hth expression in small wing pouch clones. (L) Mid third instar wing disc containing flip-out clones of brk marked by GFP, and stained for Hth and Tsh. The magnification shows a clone that fails to repress hth but has no effect on tsh expression. (M) Mid third instar wing disc containing flip-out clones of brk marked by GFP, and stained for Nubbin (Nub) and Tsh. The magnification shows a clone that has no effect on either tsh or nub expression. (N-Q) Late third instar wing disc containing Med^adro M+ clones induced by heat shock in the first instar. tsh repression is maintained in distal cells, which strongly express hth. (R-U) Late third instar wing disc containing Med^adro M+ clones induced by heat shock in the first instar. The distal limit of tsh repression coincides with the proximal limit of Dll (violet) expression.