Fig. 3. Repression of hth near the AP boundary does not require vg. (A-C) Wild-type third instar wing imaginal disc, stained for Hth (blue) and Vg (red). (A) White arrows point to the lateral hinge, where hth and vg are co-expressed at high levels. (D-I) Clones are labeled by the loss of Vg protein (red), and the loss of vgQE-lacZ and arm-lacZ expression (green). (D-F) Late third instar wing disc containing multiple vg– M+ clones, induced in the early third instar. Hth (blue) is not de-repressed in wing pouch clones near the AP boundary (a subset are outlined in white), but clones in the lateral wing pouch express Hth (yellow arrowheads). (G-I) Late third instar wing disc containing a large vg– M+ clone (white outline) induced in the second instar. The clone expresses two rings of Hth (blue), separated by a large ectopic fold.