Fig. 6. PcG genes are necessary for tsh, but not hth, repression. (A-C) Adult wing containing multiple Su(z)12^daed clones induced by vgBE::flp. Mutant clones are marked by y^– tissue, and are denoted by black arrows. (A) Note the reduction of growth along the PD axis. (B) A Su(z)12^daed clone sorts out from surrounding wild-type wing tissue. (C) A Su(z)12^daed clone differentiates bristles characteristic of more proximal cuticle. (D-M) Immunostaining of wing discs with Su(z)12^daed or Pc^– clones. Hth staining is in blue and Tsh is in red. Mutant tissue is marked by the absence of GFP. (D-F) vgBE::flp-induced Su(z)12^daed clones in the wing pouch. tsh is ectopically expressed at high levels throughout much of the mutant tissue. hth is weakly expressed in a subset of tsh-expressing cells. (G-I) Like Su(z)12^daed clones, vgBE::flp-induced Pc^– clones de-repress tsh but not hth. (J-M) Expression of the distal wing fate markers Dll (J,K) and nub (L,M) is lost or reduced in Su(z)12^daed mutant tissue (yellow arrowheads).