Fig. 3. Neurite growth from Foxd1 (BF-2 in figure) deficient VT explants is not inhibited by chiasm cells. (A) Retinal explants from E14.5 Foxd1^+/+ (a,c) or Foxd1^lacZ/lacZ (b,d) embryos co-cultured with dissociated chiasm cells from Foxd1^+/+ (a, b) or Foxd1^lacZ/lacZ (c,d) embryos. Drawings at the top indicate the two different combinations: (left) RGC axons from Foxd1^+/+ VT but not DT retina are inhibited by Foxd1^+/+ or Foxd1^lacZ/lacZ chiasm cells, whereas (right) RGC axons from the VT retina of Foxd1^lacZ/lacZ embryos are not inhibited by Foxd1^+/+ or Foxd1^lacZ/lacZ chiasm cells. (B) Quantification of mix-and-match experiments shows that RGC axons from Foxd1^+/+ VT retina are inhibited by chiasm cells from either Foxd1^lacZ/lacZ or Foxd1^+/+ embryos. However, there is a loss of significant difference between VT and DT axon behaviors when retina from Foxd1^lacZ/lacZ embryos are co-cultured with Foxd1^+/+ or Foxd1^lacZ/lacZ chiasm cells. ^**P<0.005. Scale bars: 500 µm.