Fig. 2. Cholesterol is at limiting concentration in ncr-2; ncr-1 mutants. Higher cholesterol levels suppress the Daf-c phenotype of ncr-2; ncr-1, but not the other Daf-c mutants, including daf-2(e1370ts), daf-7(e1372ts) and daf-9(m540). The dauer formation of Gen 2 worms is presented here (see Materials and methods). The error bars indicate the s.d. values of binomial populations. (B) The brood size of ncr-2; ncr-1 mutant is enhanced by higher cholesterol levels. ^*P<0.0001, Kruskal-Wallis nonparametric ANOVA test. (C) The average life span (±s.d.) of recovered ncr-2; ncr-1 dauers on plates containing 5 µg/ml and 20 µg/ml cholesterol is 7.7±5.1 days and 12.9±6.2 days respectively, P<0.0001, Student's t-test. The experiments in B,C were conducted at 20°C. n2; n1 is ncr-2(nr2023); ncr-1(nr2022) and n2; d12 n1 is ncr-2(nr2023); daf-12(m20) ncr-1(nr2022).