Fig. 5. Muscle attachments sites are unaffected in Df(2R)ED3716^CG2 mutant embryos. Muscle attachment sites were analyzed in Df(2R)ED3716^CG2 mutants. Df(2R)ED3716^CG2 mutant (A-D), and control Df(2R)ED3716^CG2/CyOwglacZ embryos (E-H) were stained with anti-ßPS integrin antibodies to visualize muscle attachment sites (A,C,E,G). Embryos were double-stained with anti-myosin heavy chain (MHC) to visualize gross muscle structure (B,D,F,H). Both genotypes showed wild-type localization of ßPS integrin at muscle attachment sites (arrowheads), as well as normal muscle morphology. Mutant embryos were identified by absence of lacZ balancer (red in F and H).