Fig. 6. Overexpression of Fak56 results in embryonic muscle detachment. Wild-type embryos and embryos expressing Fak56 specifically in embryonic muscle, using the Mef2-GAL4 driver, were stained with anti-ßPS antibodies (green) to visualize integrins and Phalloidin (red) to visualize actin. Embryos overexpressing Fak56 specifically in embryonic muscle, show a potent muscle detachment phenotype (E,F, arrows), when compared with wild-type (B,C). Interestingly, in spite of obvious rounding up of somatic muscles, integrins, as monitored by anti-ßPS-integrin antibodies, were still localized at the ends of the muscles that remain attached in Fak56 overexpressing embryos (D, arrowhead), indistinguishable to wild-type embryos (A, arrowhead).