Fig. 2. Expression of the stabilized form of ß-catenin leads to an ectodermal phenotype. (A,B) Histological sections of E6.5 wild-type and mutant embryos that were Hematoxylin and Eosin-stained. (C,D) Immunohistochemical analysis of sections from E5.5 embryos to detect ß-catenin. (E,F) Immunohistochemical analysis of sections from E6.5 embryos to detect ß-catenin. ß-catenin was localized at the cell membrane in wild-type embryos, whereas in the mutant epiblast, ß-catenin was enhanced and distributed throughout the cells. All embryos are oriented with the embryonic part to the left. EE, embryonic ectoderm. Arrow indicates thickened visceral endoderm in the mutant. Scale bar: 100 µm.