Fig. 7. Overexpression of tsg1 RNA causes dorsalization. (A-E) Whole-mount in situ hybridization with pax2.1 (arrowhead), krox20 (brackets) and myod reveals expansion of all three markers in embryos injected with 200 pg tsg1 RNA (C-E) relative to wild type (A,B). (B,D) More posterior views of embryos shown in A and C, respectively. (C,D) Mildly dorsalized and (E) moderately dorsalized embryos. (F-I) At 1 dpf, embryos injected with 100 pg tsg1 RNA show a range of dorsalized phenotypes. (F) Uninjected sibling. (G) Class 1 dorsalization, arrowhead indicates gap in ventral tail fin. (H) Class 2 dorsalization, inset shows duplication in fin fold tissue seen in 50% of class 1 and class 2 embryos. (I) Class 3 phenotype.