Fig. 2. GFP-Nos distribution during early to middle stages of oogenesis. (A-C) Fixed 6 x gfp-nos egg chambers, with GFP-Nos visualized directly and the actin cytoskeleton labeled red with rhodamine-phalloidin. (A) GFP-Nos (green) expression is strong in regions 1 and 2 of germarium (g, inset) and in the nurse cells during stages 5-8. In addition to forming cytoplasmic particles, GFP-Nos is enriched at the periphery of the nurse cell nuclei (arrows). GFP-Nos can also be detected in the oocyte during midoogenesis (arrowhead). (B) Stage 9 egg chamber with high levels of GFP-Nos in the nurse cells (nc) and perinuclear enrichment. Lower levels of GFP-Nos are present in the oocyte (oo). (C) Stage 10 egg chamber with loss of perinuclear GFP-Nos localization as nurse cells prepare for dumping. (D) High-magnification image of nurse cell nuclei from a stage 5 6 x gfp-nos egg chamber showing (i) DNA stained with Hoescht's dye; (ii) GFP-Nos; (iii) anti-Vas immunofluorescence; (iv) merge of i-iii. GFP-Nos colocalizes with Vas at the nuclear periphery. A similar distribution of GFP-Nos is observed in gfp-nos:tub3'UTR egg chambers at the stages shown in A-C.