Fig. 3. GFP-Nos distribution at late stages of oogenesis. (A-C) Lower power images of the anterior region of fixed 6 x gfp-nos egg chambers used for staging. (D-F) Higher power images of the posterior region of egg chambers shown in A-C. (A,D) Stage 11 egg chamber, with transfer of GFP-Nos produced in the nurse cells to the oocyte during nurse cell dumping. (B,E) Stage 12 oocyte, with GFP-Nos distributed uniformly throughout the ooplasm. (C,F) Stage 13 oocyte, in which GFP-Nos has disappeared from the anterior and a gradient of GFP-Nos is observed emanating from the posterior pole. This gradient is also detected in 2 x gfp-nos oocytes (see Fig. S1 in the supplementary material). The actin cytoskeleton is labeled with rhodamine-phalloidin (red) in all panels.