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Fig. 7. Distal cell neurons intermingle with lamina glia in Robo family knockdowns. (A,B) Lateral view (anterior at bottom). IPC neuroblasts and distal cell neurons are visualized with anti-Fas3 (red), c155:GFP is most strongly observed in IPC neuroblasts and photoreceptors (green), and neuronal nuclei are visualized with anti-Elav (blue). (A) c155-Gal4;UAS-GFP. (B) Distal cell neurons enter the lamina in c155-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animals (arrow), reaching anterior edge of lamina (arrowhead). (C-F) Animals express GFP (green) under control of Sca-Gal4, labeling the IPC, distal cell neurons and medulla cortex. Neuronal nuclei are visualized with anti-Elav (blue). (C,D) Photoreceptor axons are visualized using anti-Chaoptin (red). (C) Sca-Gal4;UAS-GFP animal. (D) Distal cell neurons enter the lamina in Sca-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animals (arrow). (E,F) Lamina glia are visualized using anti-Repo (red). Distal cell neurons intermingle (arrows) with lamina glia in Robo family knockdown animals. (E) Sca-Gal4;UAS-GFP;UAS-RoboRNAi,UAS-Robo3RNAi;UAS-RoboRNAi,UAS-Robo3RNAi animal. (While Sca-Gal4;UAS-RoboRNAi;UAS-Robo3RNAi animals had no defects, animals containing two copies of both UAS-RoboRNAi and UAS-Robo3RNAi had modest defects, consistent with overlapping roles of Robo family members.) (F) Sca-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animal. dcn, distal cell neuron progeny; IPC, inner proliferation center; lam, lamina; medc, medulla cortex.