Fig. 1. Phenotype of maternally rescued mau-2 animals. (A) Detail of the dorsal cord and the vulval muscles in young adult worms carrying the reporter unc-31::gfp used to visualize the nervous system and the vulval muscles. The dorsal cord is normal in the wild type (a), defasciculated in mau-2(qm5) mutants as indicated by the arrow (b), and normal in maternally rescued mau-2(qm5) animals (c). The vm1 vulval muscles (black asterisks) are normal in the wild type (e), and at least one of the vm1 is abnormally placed in mau-2(qm5) mutants and in maternally rescued mau-2(qm5) animals, as indicated by the arrow (f). (B) Quantification of the phenotype in maternally rescued mau-2(qm5) animals, compared with the wild type and mau-2(qm5) mutants (n=50-200). Both the placement of the soma and the projection of the axon of the AVM neurone are completely maternally rescued. The axon of HSN could not be scored in mau-2 mutants (red asterisk), as tph-1::gfp is not expressed in HSNs for unknown reasons, while it is still expressed in the NSMs (Sze et al., 2000).