Fig. 2. Positional cloning of mau-2. (A) Genetic markers used to map mau-2. (B) Cosmids and subclones of C09H6 used for rescue assays (rescuing clones in bold). (C) Genomic structure of mau-2 and the lesions of the four mau-2 alleles. (D) An alignment of the predicted amino acid sequence of C. elegans MAU-2 with its homologues from D. melanogaster (CG4203-PA), F. rubripes (SINFRUP00000081396), M. musculus (NP_083269) and H. sapiens (KIAA0892). Identical residues in all five species are shown in dark grey; identical residues in three or four species are shown in light grey. The mau-2(qm4) mutation affects the conserved Gly residue (indicated by an asterisk). The C. elegans protein is about 25% identical to the vertebrate and the fly proteins. The sequence in the fish is 95% identical to human MAU-2, and the mouse sequence is more than 99% identical to human MAU-2.