Fig. 5. mau-2 functions cell autonomously for the guidance of AVM. (A) Constructs used to express mau-2(+) in different tissues. The expression of each transgene was monitored with gfp. Rescue of the locomotion, egg laying and larval development are indicated. (B) Phenotype of the axon of AVM in mau-2(qm160)-expressing mau-2(+) in different tissues. AVM guidance was examined with mec-4::gfp. Four independent transgenic lines were scored (n>200 for each construct). Light grey indicates that the axon projected anteriorly; dark grey, posteriorly. The AVM guidance defect observed in mau-2 is rescued by expression of mau-2(+) in the nervous system and in the mechanosensory neurones alone. Error bars indicate standard error of the proportion. The effect of expressing mau-2(+) under these different promoters was also examined in the mau-2(+) background. The transgenic animals were completely wild type for locomotion and egg-laying, as well as for the guidance of the AVM axon (n>137 transgenic animals from three lines scored for each construct, data not shown).