Fig. 5. Molecular epistasis of Notch, EMC and EYA. (A-H) Loss-of-function clones of N^55E11 (A,B,G,H,), emc¹ (C,D) or eya^54C2 (E,F) mutant cells marked with GFP (A,C,G), or anti-EYA (E, green) and stained with either anti-EMC (A,B,E,F; red in A,E) or anti-EYA (C,D,G,H; red in C,G). The dashed lines indicate clones of mutant cells. (I-L) FLP-OUT clones of cells expressing activated Notch (UAS-N34A: designated N-Act) in two separate stage-10 egg chambers, marked with GFP to show N34A-expressing cells in green (I,K) and stained with anti-EMC (I,J; red in I) or with anti-EYA (K,L; red in K). The arrows indicate cells overexpressing EMC (I,J) or EYA (K,L). The dashed lines (I,J) and arrows (K,L) indicate cells overexpressing UAS-N34A. Scale bar: 10 µm.