Fig. 6. The effect of EMC overexpression on polar cell differentiation and on expression of EYA. (A-D) Two examples of fused egg chambers in which EP3620 is overexpressed using FLP-OUT Gal4, stained with anti-Fas3 antibody (A,C; red) and DAPI (A-D; blue in A,C), possessing three polar cell groups (arrows) rather than four, and having either a layer of cells between the fused egg chambers (B, arrowheads) or no such layer (D). (E-K) EP3620 (E,F,H-K) or UAS-LacZ (G) overexpressed using Upd-Gal4. (E,F) An ovariole (E) or two egg chambers in a different ovariole (F) stained with DAPI, showing an egg chamber with the oocyte positioned toward the anterior rather than the posterior of the ovariole (arrows). (G,H) Portions of the anterior end of an egg chamber (G) and the posterior end of one egg chamber (H, left) and anterior end of its neighbor (H, right) expressing UAS-LacZ (G) or EP3620 (H), and showing expression of EYA (blue), and the polar cell marker PZ80 (red). The arrowheads indicate the shape of the anterior end of the egg chamber and the position of the expected polar cell. The arrow indicates the region magnified in I-K. (I-K) EYA expression (I), polar cells (J) and Gal4-expressing cells (marked with GFP, K). The arrows indicate a polar cell expressing Gal4 (and presumably EMC) but not EYA. The arrowheads indicate a cell expressing Gal4 and EYA but not PZ80. Scale bars: in A, 25 µm in A-F; in G, 25 µm in G,H; in I, 10 µm in I-K.