Fig. 4. Expression of the binding domains of VAB-8 and UNC-51 in the CANs disrupted posteriorly directed axon outgrowth. (A) At the top is a schematic representation of the transgene that expresses the VAB-8 peptide in the CANs. A cDNA containing this UNC-51-binding domain of VAB-8 was fused in frame to a GFP cDNA and driven from the ceh-23 promoter. The fluorescence photomicrograph shows a wild-type larva that carries this transgene. The arrowhead indicates the position of the CAN cell body, and the arrow indicates the end of its truncated posterior axon. The scale bar represents 20 µm. (B) The distribution of axon termination positions in wild type, mutants and animals that expressed VAB-8 and/or UNC-51 peptides in the CANs. The vab-8 allele used was ev411; the unc-14 allele used was e866. See Fig. 3 for quantification of axon phenotypes and statistical analysis.