Development -- Lai and Garriga 131 (23): 5991 Figure IG6

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Fig. 6. UNC-51 autophosphorylation. (A) Autoradiograph of samples from in vitro kinase assays. FLAG-tagged wild-type and mutant UNC-51 proteins were immunoprecipitated from COS cell extracts and incubated with [ ${gamma}$ -³²P]ATP. UNC-51(K39R) had 9% and UNC-51( ${Delta}$ AIKAI) had 0.6% of the wild-type UNC-51 autophosphorylation activity. See Materials and methods for quantification of kinase activity. (B) Loading control. The western blot was probed with anti-FLAG antibodies to detect various forms of UNC-51 proteins. Similar amounts of immunoprecipitated UNC-51 proteins were used in the kinase assay.