Fig. 4. MBD2/3 colocalizes with a subset of MI-2 proteins. (A) Establishment of a novel MBD2/3-specific antibody. Antibody specificity was confirmed by western analysis of protein extracts from 0- to 6-hour-old embryos (left panel) and confocal analysis of immunostained embryos (right panels). Significant signals for MBD2/3 were detected in control (yw), but not in MBD¹ embryos. (B) Confocal sections of embryos double immunostained with antibodies against MBD2/3 (red) and MI-2 (green). MBD2/3 was found to decorate only a limited number of nuclear foci, while MI-2 was broadly distributed over the entire nucleus. Scale bars: 5 µm.