Fig. 5. Phylogenetic relationships of danios inferred from 12S and 16S rDNA sequences. Shown is a maximum-likelihood tree (branch lengths proportional to estimated divergence). Support values are percentages from Bayesian analysis followed by nonparametric bootstrapping. Taxa in red and green were chosen for analyses of pigment pattern metamorphosis based on phylogenetic position and embryo availability. The analysis supports the division of the danios into two genera, Danio and Devario, based on morphological criteria (Fang, 2003), and is in general agreement with previous molecular analyses of fewer taxa (Zardoya et al., 1996; Parichy and Johnson, 2001). GenBank Accession numbers for 12S and 16S sequences are (top to bottom): AY707450, AY707456; U21372, U21381; AF3226h58, AF322663; AY707446, AY707452; AF322663; AY707449, AY707455; AY707447, AY707453; AF322656, AF322661; U21376, U21384; AF322659, AF322664; U21377, U21377; U21375, U21370; AY707448, AY707454; U21553, U21554; AF322660, AF322665; U21378, U21386; AY707445, AY707451. Published sequences for D. aff. tweediei and D. pulcher were excluded owing to their limited length and the resulting loss of phylogenetic resolution.