Fig. 6. Repression of the UbxB reporter following the misexpression of modified/mutated forms of Brk in the visceral mesoderm of embryos (X-gal stained), using the 24B Gal4 driver. (A) No brk transgene. At this stage, UbxB drives lacZ expression in parasegments 6-9 of the midgut mesoderm. (B) Wild-type Brk^3PF3 completely represses UbxB. (C) Brk^NLS has no effect on UbxB expression. (D,E) However, Brk^NLSW dramatically reduces, and Brk^Stop1 completely represses, UbxB expression.