Fig. 5. Maize transgene expression levels compared with wild-type AP3 levels. Total RNA collected from inflorescences of strongly rescued AP3:Si1 and AP3:Zmm16 plants, was spotted onto nylon filters and hybridized with transgene specific probes or an AP3 gene-specific probe having identical specific activities. Expression levels are arbitrarily designated by the ImageQuant software after exposure to a phosphoimager screen.