Fig. 5. Cells originating from the caudal ganglionic eminence can migrate ventrally. (A) Summary of the type of graft and the results obtained after 60 hours of incubation. (B) A caudal ganglionic eminence (CGE) transplant shows GFP-positive cells migrating ventrally in two streams: a medial stream and a lateral stream. The arrows indicate that a few cells have reached the anterior hypothalamus. (C-F) Double immunofluorescence of GFP (green) and COUP-TFI (C,D) or COUP-TFII (red); E,F) on adjacent sections of the graft shown in B. Note that all GFP-positive cells have a leading process oriented ventrally (arrows), but that only COUP-TFI is double positive for GFP (insets in C,D). ant hypo, anterior hypothalamus; LS, lateral stream; MS, medial stream.