Fig. 5. xKaiso depletion leads to premature activation of transcription of a subset of genes regulated by xDnmt1. (A) [-³⁵S]UTP incorporation detecting the activation of gene expression in wild-type (WT) and 0.5 ng KMO-injected embryos. WT and KMO-injected two-cell embryos were treated with 50 nCi [-³⁵S]UTP, cultured in parallel and collected at the same developmental stage. (B) RNA derived from wild type, 5 ng KMO and 5 ng DMO (xDnmt1 morpholino) injected embryos at stage 8 were assayed by RT-PCR for the presence of transcripts of genes (xOct-25, xBef, xDrak1) regulated by xKaiso and xDnmt1, respectively, with xODC as a loading control.