Fig. 5. tfr1a is required for erythrocyte hemoglobin production, but
multiple Tfr family members can compensate for loss of tfr1a in
cia. (A-H) Ventral views of the anterior region of
o-dianisidine stained embryos at 40 hpf. (A) Uninjected wild type.
(B) Uninjected ciaiu089. (C) Wild-type embryo injected
with tfr1a MO1 did not exhibit hemoglobinized erythrocytes. (D)
Wild-type embryo co-injected with tfr1a MO1 and tfr1a cRNA
was partially rescued. (E-H) ciaiu089 embryos injected
with cRNA of tfr1a (C), tfr1b (D), mouse tfr1 (E),
and mouse tfr2 (F) all exhibited partial rescue of hypochromia.