Fig. 1. Experimental protocols used to assay for capacitation in wild-type and GalT I-null sperm. Sperm were isolated, collected by centrifugation, and capacitated for various periods of time (starting at time 0) in either complete medium or medium deficient for specific components as described in the text. At the indicated time, aliquots of sperm were incubated either with eggs for 30 minutes (to monitor for zona binding activity), or with A23187 for 10 minutes (to monitor induction of the acrosome reaction).