Fig. 2. Wild-type and GalT I-null sperm binding to ovulated eggs is competitively inhibited by a ligand peripherally associated with the ovulated zona pellucida (ZP). (A) Inhibition of wild-type sperm binding by glycoproteins solubilized from either the matrix fraction or peripheral fraction. (B) Inhibition of GalT I-null sperm binding by the matrix and peripheral fractions. Each point represents the mean±s.e.m.; n=3 experiments. In a single experiment, the value for each data point represents the average of three determinations (for a total of nine assays per data point).