Fig. 7. Ovulated, but not ovarian, zona pellucida (ZP) glycoproteins contain a basic, WGA-reactive, 250 kDa glycoprotein. Ovarian (A) and 500 zonae equivalents of ovulated (B) zona proteins (2.5 µg) were separated by two-dimensional polyacrylamide gel electrophoresis, transferred to PVDF and visualized by staining with biotinylated-WGA. This blot is representative of five experiments. The arrow indicates a 250 kDa, basic, WGA-reactive protein that is present in ovulated ZP, but not ovarian ZP. The pH gradient, the theoretical location of the matrix proteins [according to Bleil and Wassarman (Bleil and Wassarman, 1980b)], and molecular weight markers are shown.