Fig. 3. Lim1 expression in MIS signaling mutant mice. (A-D) Lateral view of the testicular region in wild-type (A), Misr2^–/– (B), Mis^–/– (C) and Wnt7a^–/– (D) male embryos at E15.75. Fragmentation of Lim1^tlz expression in the Müllerian duct is observed in wild-type embryos (arrows in A), which is suppressed in MIS signaling-deficient mutants (B-D). (E-J) Ventral view of the urogenital system in wild-type (E,G,I) and MT-hMIS^tg/+ transgenic (F,H,J) female embryos. (K,L) Ventral view of the testicular region in wild-type (K) and MT-hMIS^tg/+ transgenic (L) male embryos. Note that fragmentation of Lim1^tlz expression in the Müllerian duct is enhanced in MIS transgenic males. k, kidney (metanephros); md, Müllerian duct; od, oviduct; ut, uterus; wd, Wolffian duct. Scale bars: 250 µm in A-D; 500 µm in E-L.