Fig. 6. Cell-autonomous requirement of Lim1 for epithelium development of the female reproductive tract. (A-B) Craniofacial development of female chimeras at E18.5. XO cells derived from ES cells are stained blue by ß-gal staining. High contribution of Lim1^–/– cells in chimeras cased head truncations. (C-F) The female reproductive tract of female chimeras. XO cells derived from ES cells are stained blue by ß-gal staining and wild-type XX cells derived from blastocysts are pink by eosin staining. Lim1^–/– cells are not present in the epithelium in the uterus (arrow in D) and the oviduct (arrow in F) but present in the surrounding mesenchyme of the both tissues. In control experiments, wild-type XO cells can contribute to the epithelium of the uterus (arrow in C) and the oviduct (arrow in E). (G,H) The reproductive tract of female chimeras at E12.5. Lim1^–/– XO cells are not present in the epithelium of the developing Müllerian duct (arrow in H). Lim1^–/– XO cells are also not found in the epithelium of the Wolffian duct (arrowhead in H). Scale bars: 5 mm in A,B; 50 µm in C-F; 25 µm in G,H.