Fig. 7. Expression patterns of Shh, Gli1, Ptch and Six1. (A-F) Expressions of Shh (A,B), Gli1 (C,D) and Ptch (E,F) in wild-type (A,C,E) and Six1^–/– (B,D,F) embryos at E10.5. (G,H) Expression of Six1 in wild-type (G) and Shh^–/– (H) otic vesicles at E10.5. Three pairs of wild-type and Six1^–/– embryos and three pairs of wild-type and Shh^–/– embryos were analyzed and virtually the same results obtained. d, dorsal; la, lateral. fp, floor plate; nc, notochord. Scale bars: 100 µm.